Volume 3, Issue 4, July 2015, Page: 63-67
The Anticancer Effects of Hexane Extract of Rainbow Trout (Oncorhynchus mykiss) Muscle Enriched with Vegetable Oil Brasica sp.
Poroshad Montazeri Shahtoori, Faculty of marine science and technology, Islamic Azad University North Tehran Branch, Tehran, Iran
Mozhgan Emtyazjoo, Faculty of marine science and technology, Islamic Azad University North Tehran Branch, Tehran, Iran
Shahin Bonakdar, National cell Bank Department, Faculty of Pasteur Institute of Iran, Tehran, Iran
Mohammad Rabani, Faculty of marine science and technology, Islamic Azad University North Tehran Branch, Tehran, Iran
Received: May 25, 2015;       Accepted: Jun. 16, 2015;       Published: Jul. 3, 2015
DOI: 10.11648/j.crj.20150304.11      View  4337      Downloads  154
Background: As Canola oil (Brasica sp.) has beneficial compositions of essential fatty acids like saturated fatty acids, mono unsaturated fatty acids (MUFA) and poly unsaturated fatty acids (PUFA), practically it use in many experimental purposes. There are considerable amounts of oleic acid which is mono unsaturated fatty acids in canola oil that caused a good resistance to thermal processes such as deep frying. Rainbow trout is the main fish species farmed in about 85% of the total rate allocated to producing aquaculture fish in Iran. Rainbow trout has found in food baskets of many people due to its high nutritional value. Methods: in this study the hexane extracts of fish muscle isolated with purity of 250, 50, 10, 5 and 0.5 μg/ml at regular intervals of 24, 48 and 72 hours to determine the effects of hexane extracts on parameters which are associated with the survival of skin cancer cells A431 by MTT method in vitro comparison. Results: The effect of various concentrations of hexane extracts from fish muscle showed that the extracts of muscle at varying time intervals in hexane extracts administration had different effects on mortality of A431 cancer cells. Increasing of hexane extracts had significantly decreased the carcinogenic cells. The concentrations of hexane extract in 250 μg/ml at 24 hours were killed 50 percent of skin cancer A431cell lines. The addition of Brasica sp. vegetable oil as a dietary supplement is required to fish diet rations. Conclusion: In the present study the hexane extract inhibit the growth of cancer cells where the extract can be used as an anticancer agent for the operation of this lineage.
Cancer, Rainbow Trout (Oncorhynchus mykiss), Canola Oil (Brasica sp.), MTT Assay
To cite this article
Poroshad Montazeri Shahtoori, Mozhgan Emtyazjoo, Shahin Bonakdar, Mohammad Rabani, The Anticancer Effects of Hexane Extract of Rainbow Trout (Oncorhynchus mykiss) Muscle Enriched with Vegetable Oil Brasica sp., Cancer Research Journal. Vol. 3, No. 4, 2015, pp. 63-67. doi: 10.11648/j.crj.20150304.11
Abo ElMaati, M.F., Labib, S.M., Al-Gaby, A.M.A. & Ramadan, M.F., 2012. Antioxidant Properties of Different Extracts from Five Medicinal Plants. Agricultural biochemistry department, Faculty of Agriculture, Zagazig University, Egypt., Vol. 39, No. (4) : 1-13.
Beigmohammadi, Z , Maghsoudlou, Y., Sadeghi Mahoonak, A.R. & Safafar, H., 2009. Fatty acids Profile, Physicochemical Properties and Mineral Contents of Oil of Major Canola Varieties Grown in (Golestan Province). J. Agric. Sci. Natur. Resour., Vol. 16(2).
Buiatti, E.M.D,.Msc., 1997.The role of chemoprevention in cancer control. Salud Publica de Mexico, 39(4):310-317.
Challem, J. J., 1997. Beta-carotene and other carotenoids: Promises Failures,and a new vision. Jornal of orthomolecular Medicine Vol.12,No.1.
Chiddambara, K.N., Vanitha, A., Rajesha, j., Mahadeva Swamy, M., Sowmya, P.R., Gokare, A. Ravishankar, 2004. Invivo antioxidant activity of carotenoids from Dunaliella salina – agreen microalga, Life Science., Vol. 76, PP:1381-1390.
Emtyazjoo, Mo., Moghadasi, Z., Rabbani, M., Emtyazjoo, Ma., Samadi, S. & Mossaffa, N., 2012. Anticancer effect of Dunaliella salina under stress and normal conditions against skin carcinoma cell line A431 in vitro. Iranian Journal of Fisheries Sciences, 11(2); pp: 283-293.
Jafari, S.M.A., Rabbani, M., Emtyazjoo, M. & Piryaei, F., 2014. Effect of dietary Spirulina platensis on fatty acid composition of rainbow trout (Oncorhynchus mykiss) fillet. Aquacult Int, DOI 10.1007/s10499-013-9748-0.
Khajehpor, M.R., 1991. Production of Industrial Vegetables. Sanati Sfahan Jahad Daneshgahi Press, 370p. (In Persian).
Mohammed, M. & Mohd, M., 2012. Production of Carotenoids (Antioxidants / Colourant) in Spirolina platensis in response to INDOLE ACETIC ACID (IAA). International Journal of Engineering Science and Technology. Vol. 3 No. 6 June 2011.
Parker, T.D., Adams, D.A., Zhou, K., Harris, M., Yu, L., 2003. Fatty acid composition and oxidative stability of cold pressed edible seed oils. J. Food Sci. 68:1240-1243.
Poppel, G.V. & Goldohm, R.A., 1995. Epidemiologic evidence for β-carotene and cancer prevention. Jour. Am J Clin Nutr, Vol.62:1393S-402S.
Sadegi-aliabadi, H., Asghari, G., Mostafavi, S.A., Esmaeili, A.,2009. Solvent optimization on Taxol extraction from Taxus baccata L., using HPLC and LC-MS Daru; 17(3): 192-198.
Schwartz, J. ; Shklar, G. 1988. Prevention of experimental oral cancer by extracts of spirulina-dunaliella algae. Harvard School of Dental Medicine. Pub. In Nutrition and Cancer 11, 127-134. 1988. USA.
Siavash, B., Carapetian, J. & Zare, S., 2005. Studying on lipid content and fatty acids in some varieties of colza (Brassica napus L.). Pajouhesh & Sazandegi, No:67; pp: 95-101. (In Persian).
Stryker, W.S., Stampfer, M.J., Stein, E.A., Kaplan, L., Louis, T.A., Sober, A., Wlllett, W.C., 1990. Diet, plasma levels of beta-carotene and alpha-tocopherol, and risk of malignant melanoma., Channing Laboratory, Boston, MA 02115, Am J Epidemiol; 131:597-611.- Tim et al., 2007
Yang, C., Liu, H., Li, M., Yu, C., Yu, G., 2013. Treating urine by Spirulina platensis. Acta Astronautica 63, 1049–1054.
Zakir Khan, Pratiksha Bhadouria and P.S. Bisen, 2005. Nutritional and Therapeutic Potential of Spirulina. Bentham Science Publishers Ltd. Current Pharmaceutical Biotechnology, No. (6): 373-379.
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