A Novel Diagnostic Marker: Proteasome LMP2/1i-Differential Expression in Human Uterus Mesenchymal Tumor
Takuma Hayashi,
Akiko Horiuchi.,
Nobuo Yaegashi.,
Tanri Shiozawa.,
Susumu Tonegawa.,
Ikuo Konishi
Issue:
Volume 1, Issue 1, May 2013
Pages:
1-6
Received:
8 April 2013
Published:
2 May 2013
Abstract: Uterine leiomyosarcoma (LMS) develops more often in the muscle tissue layer of the uterine body than in the uterine cervix. The development of gynecologic tumors is often correlated with female hormone secretion; however, the development of uterine LMS is not substantially correlated with hormonal conditions, and the risk factors are not yet known. Importantly, a diagnostic-biomarker, which distinguishes malignant LMS from benign tumor leiomyoma (LMA), is yet to be established. Accordingly, it is necessary to analyze risk factors associated with uterine LMS, to establish a treatment method. Proteasome LMP2/1i-deficient mice spontaneously develop uterine LMS, with a disease prevalence of ~40% by 14 months of age. We found LMP2/1i expression to be absent in human LMS, but present in human LMA. Therefore, defec-tive-LMP2/1i expression may be one of the risk factors for LMS. LMP2/1i is a potential diagnostic-biomarker under the combination of candidate molecules for uterine mesenchymal tumors, especially uterine LMS, and may be a tar-geted-molecule for a new therapeutic approach. (160 words)
Abstract: Uterine leiomyosarcoma (LMS) develops more often in the muscle tissue layer of the uterine body than in the uterine cervix. The development of gynecologic tumors is often correlated with female hormone secretion; however, the development of uterine LMS is not substantially correlated with hormonal conditions, and the risk factors are not yet known....
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Reactive Changes of Cytophysiological Properties, Molecular-Biological Profile and Functional Metabolic Status of Cells in Vitro with Different Sensitivity to Cytostatic Agents Under the Influence of Magnetic Fluid
Lukianova N Yu.,
Naleskina L A.,
Bezdenezhnykh N O.,
Kunskaya L M.,
Demash D V.,
Yanish Yu V.,
Todor I N.,
Chekhun V F.
Issue:
Volume 1, Issue 1, May 2013
Pages:
7-14
Received:
14 May 2013
Published:
10 June 2013
Abstract: The peculiarities of changes of phenotype properties in MCF-7 human breast cancer cells with different sensitivity to cytostatic agents under the influence of nanoparticles of magnetic fluid were studied. It is defined that the biggest manifestations of magnetic fluid effect both by its essence and direction are observed in cells of resistant MCF-7/Dox line and parental MCF-7/S line in comparison with MCF-7/CP (cisplatin-resistant cell line). In particular, these are more apparent cytotoxic effects and ability to colony-formation, increase of adhesive properties, decrease of invasiveness and proliferative activity, changes of surface charge density and electrokinetic potential as well as lipid composition of cells. The defined differences between MCF-7 cells with different sensitivity to cytostatic agents according to phenotypic manifestations of correlations (magnetic fluid effect – reactive response) are evidence of the individual peculiarities of free radicals formation in cells and development of oxidative stress. The indicated phenomenon of selective reactive changes in cells by effect of magnetic fluid is necessary to take into account in development of systems of directed transport of antitumor drugs.
Abstract: The peculiarities of changes of phenotype properties in MCF-7 human breast cancer cells with different sensitivity to cytostatic agents under the influence of nanoparticles of magnetic fluid were studied. It is defined that the biggest manifestations of magnetic fluid effect both by its essence and direction are observed in cells of resistant MCF-7...
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